IDENTIFIKASI MORFOLOGI DAN MOLEKULER SEBAGAI
DASAR PENGEMBANGAN TANAMAN PORANG - CORE CORE 🇺🇦 make metadata, not war Services Services overviewExplore all CORE services Access to raw data API Dataset FastSync Content discovery Recommender Discovery OAI identifiers OAI Resolver Managing content Dashboard Bespoke contracts Consultancy services Support us Support us MembershipSponsorship Community governance Advisory Board Board of supporters Research network About About us Our mission Team Blog FAQs Contact us research oai:generic.eprints.org:10925/core478 IDENTIFIKASI MORFOLOGI DAN MOLEKULER SEBAGAI DASAR PENGEMBANGAN TANAMAN PORANG Authors Dwi Harjoko Amalia T Sakya Muji Rahayu Publication date January 1, 2011 Publisher AbstractThe research aims to get the kind of Amorphophallus oncophyllus having superior properties and produce high level of glucomannan and starch so appropriate to be cultivated based of morphological and molecular characters, get the most influence significant of agronomy variable on glucomannan and starch level. Field research was conducted in the Central Java and East Java. Laboratory research was conducted on Plant Physiology and Agricultural Biotechnology Laboratory Faculty of Agriculture and Central Laboratory UNS Surakarta. The study was conducted from April – October 2010. Sampling is taken randomly in four areas in Hutan Bromo Karanganyar, Jumantono, Saradan dan Nganjuk. Morphological identification is done by morphological observation involved observing performance, leaves, stems, roots flowers and tuber. Identification of molecular is done with RAPD method that is began with the isolation of DNA, the quality and quantity of DNA test, and DNA polymorphism analysis. Identification agronomist variable component is determined by soil conditions, micro climate and culture technique. Analysis og chemistry content involved glucomannan and starch. Data were analyzed using description analysis, and data from electrophoresis DNA were analysed based on the appearance RAPD band pattern of DNA using cluster analysis. From the results can be concluded that (1) Amorphophallus oncophyllus in the region Hutan Bromo Karanganyar, Jumantono, Saradan dan Nganjuk has same of the performance, leaves, stems, roots, flower and tuber; (2) Identification of molecular A. oncophyllus used three primer i.e. SBH 12, SBH 13, SBH 19 ; (3) Glucomannan and strach content are influenced total N, K exchange, Organic C, organic material, pH, C/N ratio, microclimat and culture technique. (4) Highest content of glucomannan and starch is A. oncophyllus from Saradan and lowest content of glucomannan and starch is A. oncophyllus from Hutan Bromo Karanganyar. Key word: A. oncophyllus, morphological, moleculer, glucomanan, strach Penelitian bertujuan mendapatkan jenis porang yang mempunyai sifat unggul dan menghasilkan kadar glukomanan dan pati tinggi sehingga sesuai untuk dibudidayakan berdasarkan karakter morfologi dan molekuler serta mendapatkan variabel agronomis yang paling berpengaruh terhadap pembentukan pati dan glukomanan. Penelitian lapangan dilaksanakan di Jawa Tengah dan Jawa Timur. Penelitian laboratorium dilakukan di Laboratorium Fisiologi Tanaman dan Bioteknologi Pertanian Fakultas Pertanian dan Laboratorium Sentral UNS Surakarta. Penelitian dilaksanakan mulai bulan April - Oktober 2010. Pengambilan sampel dilakukan secara acak di empat wilayah yaitu Hutan Bromo Karanganyar, Jumantono, Saradan dan Nganjuk. Identifikasi morfologi meliputi habitus, daun, batang, akar, bunga dan umbi. Identifikasi molekuler dimulai dengan isolasi DNA, kualitas dan kuantitas tes DNA, dan analisis polimorfisme DNA. Identifikasi komponen variabel agronomis ditentukan oleh kondisi tanah, iklim mikro dan tehnik budidaya yang diterapkan. Analisis kandungan kimia umbi porang meliputi glukomanan dan pati. Data dianalisis menggunakan analisis deskripsi, sedangkan data elektroforesis DNA dianalisis berdasarkan pola DNA dengan menggunakan cluster analysis. Dari Hasil penelitian dapat disimpulkan bahwa (1) Tanaman porang di daerah Hutan Bromo Karanganyar, Jumantono, Saradan dan Nganjuk mempunyai bentuk habitus, daun, batang, akar dan warna umbi yang sama, (2) Identifikasi molekuler untuk tanaman porang menggunakan 3 primer yaitu SBH 12, SBH 13, SBH 19; (3) Kandungan glukomanan dan pati umbi porang dipengaruhi oleh kondisi lingkungan baik tanah (N total, K tertukar, C organik, bahan organik, pH dan C/N rasio), iklim mikro dan tehnik budidaya. (4) Kandungan glukomanan dan pati tertinggi terdapat pada umbi porang dari Saradan, sedangkan terendah dari daerah Hutan Bromo Karanganyar. Kata kunci: porang, morfologi, molekuler, glukomanan, pat Article PeerReviewed S Agriculture (General) Similar works Full text Open in the Core reader Download PDF Sebelas Maret Institutional RepositoryProvided a free PDF oai:generic.eprints.or... Last time updated on 5/17/2014View original full text link This paper was published in Sebelas Maret Institutional Repository. Having an issue? Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request. Report CORE is a not-for-profit service delivered by The Open University supported by CORE Members. Product Services Membership Sponsorship Organisation About us Governance Team Data providers Support Terms FAQs Blog Contact us Writing about CORE? Discover our research outputs and cite our work. COREAccessibilityCookiesPrivacy
